Fig. 7
Metabolic phenotypes of Wy-offspring during high-fat diet (HFD) feeding. a Experimental protocol of Veh-offspring and Wy-offspring fed HFD diet, which are referred to as Veh-HFD and Wy-HFD, respectively. The gray-shaded box indicates the period of maternal administration of Wy or Veh. b, c Body weight changes (b) and total food intake during HFD feeding (c) (n = 11 per group, statistics by two-way ANOVA with repeated measures). d Tissue weight of Wy- and DMSO (Veh)-treated HFD mice at 14W (n = 11 per group). e Hematoxylin and eosin (HE) staining (top, representative image of ten individuals per group) and quantification of adipocyte diameter (bottom) of eWAT. Histograms of adipocyte diameter (bottom left). Horizontal lines with bilateral squares indicate interquartile range (IQR). Arrows indicate the median values (numbers above the horizontal lines) of Veh-HFD and Wy-HFD. Statistical analysis (bottom right) of mean adipocyte diameters are shown. Scale bar = 100 µm (n = 10 per group). f Bisulfite-sequencing analysis (left, representative data of three independent experiments) and graphical presentation of statistical analysis (right, n = 4–5 per group) of Fgf21 in Wy-HFD and Veh-HFD at 4W and 14W. g Hepatic Fgf21 mRNA expression in Wy-HFD and Veh-HFD at 14W. (n = 10 per group). h Circadian variation of serum FGF21 concentrations. ZT, zeitgeber time (n = 11 per group). i Relative mRNA expression of Egr1, c-fos, Hsl, and Atgl in eWAT (n = 10 per group). Statistics by unpaired Student’s t-test otherwise indicated. Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; N.S., not significant vs. Veh-HFD
