Fig. 8
Analysis of FGF21-deficient (KO) mice. a Experimental protocol of maternal administration of Wy or DMSO (Veh) to FGF21-KO dams during the late gestation (e14–e18) and lactation periods (D2–D16). Offspring derived from Wy- or Veh-administered dams are referred to as Wy-FGF21-KO and Veh-FGF21-KO, respectively. These offspring were treated with HFD diet for 10 weeks in adulthood (from 4W to 14W). b Body weight changes during HFD feeding (n = 7–9 per group, statistics by two-way ANOVA with repeated measures). c Tissue weight of Wy- and Veh-FGF21-KO at 14W. d Serum FGF21 levels of Wy- and Veh-FGF21-KO at 14W. e Hematoxylin and eosin (HE) staining (top, representative image of 7–9 individuals per group) and quantification of adipocyte diameter (bottom) of eWAT. Histograms of adipocyte diameter (bottom left). Horizontal lines with bilateral squares indicate interquartile range (IQR). Arrows indicate the median values (numbers above the horizontal lines) of Veh-FGF21-KO and Wy-FGF21-KO. Statistical analysis (bottom right) of mean adipocyte diameters ± SEM are shown. Scale bar = 100 µm. f Relative mRNA expression of Egr1, c-fos, Hsl, and Atgl in eWAT, n = 7–9 per group. Statistics by unpaired Student’s t-test otherwise indicated. Data are expressed as mean ± SEM. N.S., not significant vs. Veh-FGF21-KO
