Fig. 1
From: A human endothelial cell-based recycling assay for screening of FcRn targeted molecules
The HERA protocol. a HMEC1-hFcRn cells are seeded and grown until confluent. b Cells are washed and starved for 1 h using HBSS (pH 7.4). c The protein of interest is diluted in HBSS (pH 7.4 or 6.0) and added to cells and incubated for 4 h. d Medium is removed and cells are extensively washed with ice cold HBSS (pH 7.4) before the cells are lysed or e pre-heated HBSS (for 4 h incubation) or growth medium (for overnight incubation (ON)) is added, incubated and samples collected. f Cells are extensively washed with ice cold HBSS (pH 7.4) and lysed. g The collected samples are analysed in ELISAs specific for IgG or albumin
