Table 1 KD values derived from MicroScale Thermophoresis analysis
From: A human endothelial cell-based recycling assay for screening of FcRn targeted molecules
KD ± s.d. (nM) pH 5.5 | KD ± s.d. (µM) pH 7.4 | Fold difference compared with WT pH 5.5 | Fold difference compared with WT pH 7.4 | |
|---|---|---|---|---|
hIgG1 variants | ||||
WT | 964.3 ± 128.0 | >60 | ||
YTE | 166.4 ± 20.1 | >20 | 5.8 | >3 |
LS | 43.6 ± 8.3 | 7.0 ± 2.0 | 22.0 | >9 |
KF | 53.4 ± 10.4 | 3.3 ± 0.9 | 18.0 | >18 |
YTE/KF | 9.3 ± 5.3 | 0.8 ± 0.2 | 107.0 | >75 |
HSA variants | ||||
WT | 520.5 ± 96.5 | NA* | ||
K573P | 49.6 ± 8.5 | >70 | 10.0 | NA |
