Fig. 3

EC-specific SOD3 expression is sufficient to increase Doxo effect. a Conditional expression allele for the SOD3-IRES-GFP gene at the ROSA26 locus. b SOD3 (green) and CD31 (red) detection in sections from tumors grown in Cre-expressing and Cre⁻ mice after tamoxifen induction. c LLC tumor growth kinetics in SOD3^EC-Tg and control (Cre⁻) mice. Arrows indicate tamoxifen and Doxo treatments (n = 10/group). d Doxo quantification in extracts of tumors dissected at day 16 from control and SOD3^EC-Tg mice (n = 15 or 17 mice/group). e, f Images and quantification of lectin-FITC-perfused and CD31-stained vessels in control and SOD3^EC-Tg tumors (0.7–1.2 cm³) dissected at day 20. g CD31 staining of control and SOD3^EC-Tg tumors at end point. h–l CD31⁺ structure density (h), mean area covered by CD31⁺ structures (i), mean vessel length (j) and diameter (k), and the number of vessel branches (l), determined in images as in g. m CD31 (green) and VEC (red) staining of tumors grafted in SOD3^EC-Tg and control mice. Bottom panels show red staining in a magnified area. n Quantification of the VEC area in CD31⁺ structures from images as in m. o Vessel permeability determined by FITC-lectin and Texas Red-dextran in tumors dissected at day 17 from control and SOD3^EC-Tg mice. For b, e–o, 10–20 images were analyzed from 5 mice/group; *p < 0.05, **p < 0.01, two-tailed Student’s t-test. Bar, 50 μm