Fig. 3

Genetic variation reveals sequences that directly regulate GATA1 chromatin occupancy. a Sequence logo reflecting canonical GATA1 motif and the percent impact on GATA1 binding intensity associated with discSNPs at various positions and to particular alternative nucleotides. Median ± 95% confidence intervals by bootstrapping. b Normalized read count in sequenced ChIP input vs GATA1 IP for deletion-containing alleles of the Bola1-proximal GATA1 peak in MEL cells. Gray points indicate deletions removed due to low Input read counts, red points indicate deletions above the read count threshold, and blue point indicates wild-type (non-deleted) MEL allele. Solid line indicates 1:1 normalized IP enrichment, and dashed lines indicate 10-fold changes in enrichment. c Aggregate effects on GATA1 IP enrichment of either full or partial deletions of the WGATAR motif or of deletions not overlapping this motif, mean ± SEM. d Aggregate effects of 1-bp or 2-bp deletions within a 2-bp sliding window across the GATA1 motif on GATA1 IP enrichment, mean ± SEM