Fig. 4
From: Precise control of SCRaMbLE in synthetic haploid and diploid yeast
Sporulation and deep sequencing analysis. a The diploid yJBD001 was sporulated and dissected on permissive agar plates, and the resulting haploid spore clones were analyzed to identify the mating type. b The crossing-over of two larger chromosome fragments was observed in the haploid spore yJBS001. The synIII YCR018C-YCR098C segment (168 kb) was exchanged with the wtIII YCR018C-YCR098C, and the YER032W-YER139C of synV (225 kb) was exchanged with the YER032W-YER139C of V. c Three inversions were observed in the haploid spore yJBS001, including one inversion in synV (YEL059W-YEL022W) and two inversions (YCR007C and YCR008W) in synIII. A translocation of YER043C-YER044C was observed in synV. d The copy numbers (duplication and deletion) of five locations in the diploid yeast yJBD001 and its spore yJBS001 were verified by qPCR. Error bars represent SD from three independent experiments
