Fig. 5
From: Precise control of SCRaMbLE in synthetic haploid and diploid yeast
MuSIC strategy for rapid evolution. a MuSIC enables the generation of chromosome diversity across a large population of cells through the iterative SCRaMbLEing and screening of yeast. Based on step-by-step screening and accumulation analysis, cells with improved production are then recovered for the subsequent cycle. High-production diploids are dissected to screen for high-level production spores. The spores are capable of mating with other synthetic haploid yeast for subsequent cycles. b Five cycles of MuSIC to screen for high-production diploids. The yJBD001, yJBD038, yJBD048, yJBD057, and yJBD069 strains were generated by SCRaMbLEing the yJBD000, yJBD001, yJBD038, yJBD048, and yJBD057 strains, respectively. c Incorporation of multiple synthetic chromosomes for SCRaMbLE. The spore from dissection of yJBD001 was mated with synX yeast haploids for the subsequent cycle of SCRaMbLE. d Deep sequencing analysis of yJBD048 and yJBD057. Duplication of YEL014C-YEL013W was observed in synV of yJBD057. In b, c, error bars represent SD from three independent experiments
