Fig. 5

Architecture-dependent turnover of the nuclear pore subunits. a Upper panel: nucleoporin half-lives mapped onto the structure of the nuclear pore complex. Nups are shown color-coded as gradient from red (short half-life) to blue (long life-life). An architectural model of the nuclear pore^{42, 43} is shown as seen from top (top panel), cut in half (middle panel), and a subcomplex scheme (bottom panel). The nucleoplasmic side is at the bottom in all cases. For each cell type, half-lives were averaged over two biological replicates, except for rare cases where only one half-life value was available, and converted to a color gradient as explained in the Methods. The median and minimum and maximum half-lives are indicated together with the color bars. Nups with non-determined half-lives are colored green. Lower panel: distributions of the reproducibly measured half-lives of the scaffold and peripheral subunits of the nuclear pore in the different cell types. Differences in the distributions of log₁₀ half-lives were assessed by Wilcoxon-rank test (significance levels were encoded as *** p < 0.001, ** p < 0.01, * p < 0.05). Center line in box plots is the median, the bounds of the boxes are the 75 and 25% percentiles i.e., the interquartile range (IQR), and the whiskers correspond to the highest or lowest respective value or if the lowest or highest value is an outlier (greater than 1.5 * IQR from the bounds of the boxes) it is exactly 1.5 * IQR b The same as upper panel in (a), but color-coded according to Nup subcomplexes. Nups of the inner ring are colored blue, of the outer (Y-complex) rings—orange, trans-membrane nucleoporins—brown, Nup205 and Nup188—green, nuclear basket nucleoporins—yellow, Nup62 subcomplex—magenta, Nup358 subcomplex—salmon, and Nup214 complex—red