Fig. 6

USP35 is a FoxM1 target gene. a A schematic of the promoter region of the human USP35 gene. The sequences and positions of a FoxM1-binding site (FBS) and a cell cycle genes homology region (CHR) in the USP35 promoter are shown. b HEK293T cells were transfected with vectors that expressed the luciferase reporter gene under the control of either the WT USP35 promoter or a mutant USP35 promoter, either alone or in combination with Myc-FoxM1. The luciferase activity was measured and normalized to the renilla luciferase activity. c, d HeLa cells transfected with Myc-FoxM1 were synchronized in prometaphase by a treatment with 100 ng/mL NOC for 18 h. c A q-PCR analysis was conducted to determine the relative expression levels of mRNAs encoding USP35, FoxM1, and Aurora B, and d a western blot analysis was performed to detect USP35 and FoxM1 target protein levels. The data in parts b and c represent the mean ± SD (*P < 0.05; **P < 0.005, t-test; n = 3)