Fig. 2

SEMA4A co-stimulates T-cell proliferation. a, b CFSE-labeled purified naive CD4⁺ T cells were cultured with immobilized suboptimal dose of anti-CD3 mAb, recombinant SEMA4A-Fc fusion protein or hIgG (Fig. 2a upper left), aggregate data were presented at the bottom. Difference between these two groups was evaluated by Student's T-test (p < 0.01603) or cultured on parental L cells or SEMA4A-expressing L cells pre-coated with a suboptimal dose of anti-CD3 mAb (Fig. 2a down left), aggregate data were presented at the bottom. Difference between these two groups was evaluated by Student's T-test (p < 0.0000218). FACS data represent one of three independent experiments, respectively. Meanwhile, CFSE-labeled purified naive CD4⁺ T cells were cultured with immobilized suboptimal dose of anti-CD3 mAb in the presence of anti-SEMA4A mAb or mIgG (b) for 5 days. T-cell proliferation was detected by CFSE dilution. Data represent one of three independent experiments. Aggregate data were presented at the bottom. Student’s T-test was used for the statistical analysis, p < 0.0000272. c CFSE-labeled purified naive CD4⁺ T cells were cultured with allogeneic CD4⁺mDC at a T:mDCs cell ratio of 5:1 in the presence of anti-SEMA4A mAb or mIgG for 7 days. T-cell proliferation was detected by CFSE dilution. Data represent one of three independent experiments. Aggregate data were presented at the bottom. Student’s T-test was used for the statistical analysis, p < 0.0000372