Fig. 6
From: Human Semaphorin-4A drives Th2 responses by binding to receptor ILT-4
SEMA4A co-stimulates CD4+ T-cell proliferation through ILT-4 receptor. a, b CFSE-labeled purified naive CD4+T cells were cultured in the presence of suboptimal dose of immobilized anti-CD3 mAb plus human SEMA4A-Fc or control hIgG with or without various concentrations of ILT-4-Fc or hIgG for 5 days a, or cultured on parental L cells or SEMA4A-expressing L cells pre-coated with a suboptimal dose of anti-CD3 mAb in the presence of various concentrations of anti-ILT-4 or mIgG for 7 days b. T-cell proliferation was detected by FACS analysis; the percentages of proliferated and non-proliferated T cells are indicated in each squares. The percentages of proliferated T cells in the indicated group were summarized as proliferation blocking curve (p < 0.01) (Student’s T-tests). Data represent one of three independent experiments
