Fig. 3
From: Solid-phase synthesis of protein-polymers on reversible immobilization supports
Properties of released PARIS-synthesized protein–polymer conjugates. Irreversible inactivation of native CT and CT−pCBMA at 50 °C. CT−pCBMA immobilized at pH 6.0 and polymerized for 2 h (red open circle), CT−pCBMA immobilized at pH 8.0 and polymerized for 2 h (purple open diamond), CT−pCBMA by solution synthesis (blue open triangle), and native CT (black closed circle). Samples were incubated in 100 mM sodium phosphate buffer (pH 8.0, 50 °C) at 3.9 μM CT and residual activity was measured over 6 h. All CT-pCBMA conjugates showed enhanced thermostability compared to native CT. CT-pCBMA immobilized at pH 6.0 with a free N-terminus showed the highest thermostability. Error bars represent standard deviation from triplicate measurements
