Fig. 2
Loss of PARP1 is synthetic lethal with HR inhibition. a U2OS cells were exposed to B02, with or without Olaparib (PARPi) or Nu7441 (DNA-PKi), and cell viability assessed by clonogenic survival assay. Error bars represent the SEM from three independent experiments. Statistical significance was determined by two-way ANOVA (NS, not significant; *** p < 0.001). b U2OS, parp1Δ, parp2Δ or parp1/2Δ cell lines were exposed to B02 and cell viability assessed by clonogenic survival assay. Error bars represent the SEM from three independent experiments. Statistical significance was determined as in a. c and d U2OS and parp1/2Δ cell lines, with or without siRNA depletion of PARP3 c, or U2OS cells with or without XRCC1 siRNA depletion d, were exposed to B02 and cell viability assessed by clonogenic survival assay. Error bars represent the SEM from three independent experiments. Statistical significance was determined as in a
