Fig. 2
In vivo electrophysiological characterization of genetic TRAPing. a Left: Schematic representation of the experimental set up for in vivo two-photon targeted recording. Middle: A representative two-photon micrograph of BFP-positive neurons from the left auditory cortex (projection of 100–400 μm under the surface of A1). Scale bar 200 μm. The 6 recorded TRAPed cells and 4 non-TRAPed cells are indicated in magenta and blue, respectively. Right: Raster plots of these 10 neurons in response to WC stimuli. b Left, representative two-photon micrographs from two neurons whose numbers correspond to the location shown in (a) (Green (Alexa488), electrode; Blue (BFP), TRAPed neuron). Scale bar, 10 μm. For each neuron we recorded its responses to pure tones at 4 intensities (middle panels) and to WCs at 3 intensities (right). Rasters in response to pure tones are shown at 68 dB SPL only. Arrows in frequency axis indicate best frequency (BF) in each cell. Frequency response area (FRA) shows mean responses across all frequencies and attenuations. Color bar indicates normalized spike count. WC responses are shown at high, medium and low intensities (H, M, L; 20 trials per intensity)
