Fig. 5
USV-TRAPed neurons in mothers are highly responsive to USVs. a Schematic representation of the four neuronal groups from three experimental conditions. We recorded from TRAPed and non-TRAPed neurons in P11–12 mothers stimulated with USV (TRAPUSV-Mother and non-TRAPUSV-Mother, respectively), from TRAPed neurons in P11–12 mothers that were not stimulated with sound (TRAPNS-Mother, green) and from neurons in P4 mothers using blind patch configuration (Blind-P4, black). Example photomicrograph of the electrode (green) and BFP signal (blue) is shown from each group. Scale bar, 10 μm. b Spiking responses to the USV stimulus. Panels are organized similar to Fig. 3b, showing data from the following groups: TRAPUSV-Mother (n = 33 neurons, N = 10 mice), non-TRAPUSV-Mother (n = 33 neurons, N = 10 mice), TRAPNS-Mother (n = 29 neurons, N = 7 mice) and Blind-P4 (n = 58 neurons, N = 7 mice). c Basic response properties to USV of all individual neurons from the four groups. Each circle represents an individual cell. USV TRAPed neurons have higher USV-evoked spike rates as compared to all control groups (*p < 0.05; post hoc Fisher’s LSD test after significant Kruskal–Wallis test). Spontaneous firing rates (p = 0.18, Kruskal–Wallis test) and the number of responded syllables (p = 0.12, Kruskal–Wallis test) were not significantly different between groups. d BF was not significantly different (p = 0.23, Kruskal–Wallis test) between the groups. e Plots of the mean (±SEM) evoked vs spontaneous spike rates of the neurons shown in (b) and (c)
