Fig. 3
From: A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
Super-resolution imaging of transiently expressed BC2-tagged proteins in chemically fixed cells. Representative dSTORM images of a a HeLa cell expressing BC2Tlamin, b U2OS cell expressing tubulinBC2T (filament width statistics in Supplementary Fig. 9a), c HeLa cell expressing BC2Tactin (coverage statistics in Supplementary Fig. 9b), d HeLa cells expressing BC2TLC3B either left untreated or treated with rapamycin. Bar charts represent the degree of clustering, given as a relative fraction of cluster points versus noise points, errors given as standard deviation (S.D.). Histograms represent cluster diameters as determined by DBSCAN analysis with a bin size of 100 nm (x-axis) plotted against relative fraction (y-axis). Full data are represented underneath the histograms as box + scatter plots with the same x-axis. The box marks the three quartiles and the whiskers mark 95% of all the data. Total number of clusters n = 342 in non-treated cells, n = 405 in treated cells, N = 3 cells for untreated cells and N = 4 cells for rapamycin-treated cells (Supplementary Fig. 11), and e HeLa cells expressing BC2TGFP-GPI. All cells were stained with bivBC2-NbAF647 (Methods section). Scale bars, images 5 µm, insets 1 µm. Crossed out rectangles mark the position of fiducial markers used for drift correction. Image reconstruction details are given in Methods section
