Fig. 4
From: A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
Visualization of endogenously expressed BC2-tagged actin labeled with bivBC2-NbAF647. a Wide-field images of chemically fixed wild-type A549 and HeLa (-wt; left panel), as well as chemically fixed A549-BC2TACTB and HeLa-BC2TACTB cells (right panel). Cells were either left untreated (0 h) or stimulated for 48 h with TGFβ (5 ng ml−1) followed by staining with phalloidinAF555 and bivBC2-NbAF647. Scale bars, 25 µm. b dSTORM image of a representative HeLa-BC2TACTB cell. Scale bars, image 5 µm, insets 1 µm. Image reconstruction details are given in Methods section. Imaging sequence taken from raw data acquisition can be found in Supplementary Movie 5, assessment of AF647 photophysics under dSTORM imaging conditions can be found in Supplementary Fig. 15
