Fig. 6

Mural cell Pparg is required for TZD-driven visceral WAT remodeling. a, b Control and Mural-Pparg^KO mice were treated as described in Supplemental Figure 10a. Body weights were measured weekly. Control + Vehicle, n = 8; Mural-Pparg^KO + Vehicle, n = 9; Control + Rosi, n = 11; Mural-Pparg^KO + Rosi, n = 19. n.s. denotes not statistically significant from two-way ANOVA. Data points represent mean + s.e.m. c Fat mass and lean mass (normalized to body weight) of control and Mural-Pparg^KO mice after 4 weeks of vehicle or rosiglitazone treatment. Control + Vehicle, n = 8; Mural-Pparg^KO + Vehicle, n = 8; Control + Rosi, n = 8; Mural-Pparg^KO + Rosi, n = 9. * denotes p < 0.05 from Student’s t-test. n.s. denotes not statistically significant. Bars represent mean + s.e.m. d Fat pad weight (normalized to body weight) of control and Mural-Pparg^KO mice after 4 weeks of vehicle or rosiglitazone treatment. Control + Vehicle, n = 8; Mural-Pparg^KO + Vehicle, n = 8; Control + Rosi, n = 8; Mural-Pparg^KO + Rosi, n = 9. * denotes p < 0.05 from Student’s t-test. n.s. denotes not statistically significant. Bars represent mean + s.e.m. e–l Representative immunofluorescence staining of Perilipin (green) and Mac-2 (red) in e–h gonadal and i–l perirenal WAT paraffin sections obtained from control and Mural-Pparg^KO mice after 4 weeks of vehicle or rosiglitazone treatment. Scale bar, 200 μm. m, n Average adipocyte size in m gonadal and n perirenal WAT from control and Mural-Pparg^KO mice treated with vehicle or rosiglitazone. Two-way ANOVA, *p < 0.05; Control + Vehicle, n = 6; Mural-Pparg^KO + Vehicle, n = 6; Control + Rosi, n = 7; Mural-Pparg^KO + Rosi, n = 8. Bars represent mean + s.e.m. o, p Number of crown-like structures (Mac-2 positive) in o gonadal and p perirenal WAT from control and Mural-Pparg^KO mice treated with vehicle or rosiglitazone. Two-way ANOVA, *p < 0.05. n = 24 randomly chosen ×10 magnification fields from six individual animals. Bars represent mean + s.e.m. q, r Relative mRNA levels of indicated rosiglitazone-induced genes in q gonadal and r perirenal WAT obtained from control and Mural-Pparg^KO mice treated with vehicle or rosiglitazone. Two-way ANOVA, *p < 0.05. n = 6 per group. Bars represent mean + s.e.m.