Fig. 3
MYC induces an alternative epigenetic program in mammary epithelial cells. a Barplot showing the distribution of MYC peaks on indicated genomic features in IMEC WT, IMEC-MYC, and M2. b Heatmap showing the dynamic behavior of MYC normalized ChIP-seq signals on MYC-bound TSS. Expression of annotated genes is reported. RPM reads per million. c Tag density plots of MYC normalized ChIP-seq signals in IMEC WT, IMEC-MYC, and M2, centered on up- (left) and downregulated (right) TSS. RPM reads per million. d GO of up- (upper panel) and downregulated (lower panel) genes bound by MYC on their TSS. e Identification of modulated enhancers among IMEC WT, IMEC-MYC, and M2. In the upper panel, the Venn diagram shows the overlap of H3K4me1 ChIP-seq peaks among different cell types. In the lower panel, identified H3K4me1-positive regions were analyzed for their enrichment in H3K27ac, leading to identification of modulated and unchanged enhancers in the reported comparisons. f Heatmap showing the dynamic behavior of H3K4me1, H3K27ac, and MYC normalized ChIP-seq signals overidentified modulated enhancers. Expression of associated genes is reported. Key relevant genes associated to different enhancers groups are indicated on the right. RPM reads per million. g TF binding motifs enrichment at enhancers activated in IMEC WT. h GO of genes associated to differentially modulated enhancers in IMEC WT and M2
