Fig. 6
MYC-induced reprogramming favors the onset of TICs. a In the upper panel, whole body imaging of NOD/SCID mice 6 weeks after injection into the sub-renal capsule of indicated cells (n = 3). In the lower panel, representative hematoxylin & eosin (H/E) and immunohistochemical staining for indicated markers on generated xenografts. Scale bar, 50 μm. b Primary xenograft tumor volume (mm3) following orthotopic injection of indicated cells in NOD/SCID mice. Data are mean tumor size ± SEM (n = 4) (**P < 0.01; Student’s t-test; IMEC-MYC-PIK3CAH1047R compared with IMEC-MYC). c Representative immunohistochemical staining of indicated markers on primary tumors generated after orthotopic injection of IMEC-MYC-PIK3CAH1047R in NOD/SCID mice (n = 4). d In the upper panel, table depicting the number of formed tumors per injected mice in a serial transplantation assay. In the lower panel, representative immunohistochemical staining of indicated markers performed on tertiary tumors. Scale bar, 50 μm. e In vivo imaging of metastasis generated in NOD/SCID mice 4 weeks after secondary tumor removal. Representative images of two mice are shown (n = 6)
