Fig. 4
From: PAN-cancer analysis of S-phase enriched lncRNAs identifies oncogenic drivers and biomarkers
SCAT1 and SCAT5 act as oncogenic drivers and prognostic markers for lung-derived and kidney-derived cancers, respectively. a Bar graph showing the significant differential expression levels of SCAT1 expressed as log2 fold change across 10 different cancer types obtained from TCGA data sets. b Kaplan–Meier plots of SCAT1 indicating overall survival of patients in LUAD (upper left panel) and LUSC (lower left panel) cancer types. The higher expression of the SCAT1 is correlated with poor overall survival. The upper and lower right panels represent the multivariate models of LUAD and LUSC cancers, respectively, derived from Cox proportional hazard analysis and associated p-values were calculated using Wald statistics. c MTT proliferation assay of A549 (LUAD) cell line depleted with two different LNA oligos targeting SCAT1. d Cell cycle profiles of control and SCAT1 KD A549 cells. e Estimation of caspase 3/7 activity in control and SCAT1 KD A549 cells. f Bar graph showing the significant differential expression levels of SCAT5 expressed as log2 fold change across five different cancer types obtained from TCGA data sets. g Kaplan–Meier plots of SCAT5 indicating overall survival of patients in KICH (upper left panel) and KIRP (lower left panel) kidney cancer. The higher expression of the SCAT5 is correlated with poor overall survival. The upper and lower right panels represent the multivariate models of KICH and KIRP cancers, respectively. h MTT proliferation assay of Caki-2 cell line 48 h post-silencing of SCAT5 using two different LNAs. i Cell cycle profiles of control and SCAT5 KD Caki-2 cells. j Estimation of caspase 3/7 activity in Caki-2 cells 48 h post-silencing of SCAT5. The significance in figures a and f was derived using Benjamini–Hochberg’s method. Note that the data presented in c–e and h–j represents the mean values of three independent experiments and statistical significance was derived using a two-tailed unpaired Student’s t-test. Data are plotted as mean ± SD (*p ≤ 0.05; **p = 0.01 ̶ 0.001; ***p < 0.001)
