Fig. 2 | Nature Communications

Fig. 2

From: Evidence that DNA polymerase δ contributes to initiating leading strand DNA replication in Saccharomyces cerevisiae

Fig. 2

Phenotypes of wild-type, pol2-4, and pol2-16 mutants. a Confocal microscope images of DAPI stained, exponentially growing yeast in the synthetic complete media, fixed with 70% ethanol. The pol2-16 cells are much bigger than wild-type and pol2-4 cells and have aberrantly distributed DNA. b Flowcytometric analysis of cell cycle progression. Yeast cells were α-factor arrested in G1 phase, then released into complete media, sampled every 20 min, fixed with 70% ethanol, stained with propidium iodide, and then analyzed with flow cytometry (Methods). 1C and 2C indicate the DNA contents. c Colony size heterogeneity in the pol2-16 outgrowths. Resuspended pol2-16 and POL2 spore colonies, were plated on solid complete media and incubated at 23 °C for 6 and 4 days, respectively

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