Fig. 3 | Nature Communications

Fig. 3

From: Integrated genomics and functional validation identifies malignant cell specific dependencies in triple negative breast cancer

Fig. 3

KIFC1 is a validated tumor addiction gene that is upregulated in TNBCs. a Primary pooled siRNA oligo validation data for KIFC1. Mean NPI are plotted and error bars represent the SEM, n = 3. b Mean NPI from three independent of HCC1143 cell line after deconvolution of pool of KIFC1 siRNAs used independently. Error bars represent SEM, n = 3. c mRNA knockdown of individual siRNAs in HCC1143 cells. Knockdown was evaluated by qPCR and represented as percentage of knockdown compared to non-silencing control from three technical replicates. d Copy number alterations of KIFC1 correlated with its gene expression across all breast cancers in the Guy’s TNBC-enriched cohort of breast cancers, (e) TCGA BRCA and (f) METABRIC data sets. g KIFC1 gene expression across the PAM50 breast cancer subtypes in the Guy’s TNBC-enriched cohort of breast cancers, (h) TCGA BRCA and (i) METABRIC data sets. Box-and-whisker plots showing median center line, 25% and 75% box limits and range of expression, non-paired two-sided Wilcoxon rank sum test; *p < 0.05, ****p < 0.0001

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