Fig. 5 | Nature Communications

Fig. 5

From: Integrated genomics and functional validation identifies malignant cell specific dependencies in triple negative breast cancer

Fig. 5

Cell lines with centrosome amplification undergo multipolar mitoses followed by mitotic catastrophe in the absence of KIFC1. a Representative immunofluorescence images of mitotic spindles in low-CA cell line (CAL51) and high-CA cell lines (HCC1143 and SKBR3) with control or KIFC1 siRNA or mock transfection. Spindles stained with Aurora A (green) and/or Eg5 (red). Scale bar is 5μm. b Mean percentage of multipolar mitotic cells. Error bars represent the SEM, n = 3. c Images of time-lapse of MDA-MB-231 cells expressing NT shRNA showing a bipolar mitosis. d Images of time-lapse of MDA-MB-231 expressing KIFC1 shRNA showing prolonged mitosis and subsequent apoptosis. e. Images of MDA-MB-231 cells with inducible KIFC1 shRNA showing an abnormal multipolar mitosis. Scale bar is 5 μm

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