Fig. 7 | Nature Communications

Fig. 7

From: Integrated genomics and functional validation identifies malignant cell specific dependencies in triple negative breast cancer

Fig. 7

Pericentrin abnormality score: a potential predictive biomarker for sensitivity to KIFC1 inhibition. a Normal breast tissue section stained for pericentrin as a centrosome marker and below, a scatter graph of DAB staining area versus DAB mean intensity. Cut-off for normal centrosome size was set at 7 µm2. b Tumor 1 with centrosomes that appear normal. Below, scatter graph of DAB staining area vs DAB mean intensity showing a PCAB score of 1.19%. c Tumor 2 with centrosomes that appear abnormal. Below, scatter graph of DAB staining area vs DAB mean intensity showing a PCAB score of 12.34%. d PCAB score vs NPI (%) upon KIFC1 silencing across panel of breast cancer cell lines. Linear regression analysis, r2 = 0.71, p < 0.05. Scale bars represent 25 μm. e. PCAB score of TMAs of cohort of 82 TNBCs. Dashed red line depicts the median PCAB score (32%) of breast cancer cell lines sensitive to KIFC1. f Kaplan–Meier curves illustrating the duration of recurrence free survival according to a 20% PCAB cut-off. Wald test, p < 0.05, hazard ratio (HR) = 1.95, confidence intervals (CI) = 1–3.89

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