Fig. 5
From: A loop region of BAFF controls B cell survival and regulates recognition by different inhibitors
Restoration of mature B cells in BAFF E247K knock-in mice treated with cross-linking anti-mBAFF antibodies. Cohorts of BAFF E247K knock-in mice received control (EctoD1) or BAFF cross-linking antibodies (Sandy-5 or 5A8) weekly for 6 weeks, then spleen and lymph nodes were analyzed for the presence of mature B cells. Groups of untreated wild type (WT) and BAFF knock-out (KO) were analyzed in parallel. a Flow cytometry analysis of spleen (top dot plots) and lymph nodes (bottom dot plots). Cell populations analyzed are shown on the left. imB: immature B cells; matB: mature B cells. b Same as panel a, but showing number of B cells in spleens of 6 mice per group (5 mice for KO). Mean ± SEM. For statistics, see panel d. c Same as panel b, but showing B to T cell ratio in lymph nodes. For statistics, see panel d. Experiment of panels a–c was performed once in this format (and once with 5A8 in two ki mice, looking at kinetics of successful B cell restoration in blood). d Results of one-way Anova followed by Bonferroni multiple comparison test for mature B cells in the spleen (lower left half) and B/T cell ratio in lymph nodes (upper right half). NS = not significant: p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Panels b–d: results of statistical test should be considered with caution because although most measures were distributed normally, the assumption of equal variance between groups was not met. See also Supplementary Table 3
