Fig. 5

RNA-seq analysis of bacterial resistance. MDR A. baumannii 10073 was treated with imipenem or pEt_20 for 30 passages at 0.5× MIC that was measured at each passage (treatment duration of each passage: 18 h). a A Venn diagram depicting the overlap of differentially regulated genes either upon pEt_20 or imipenem treatment, relative to untreated controls. The numbers inside the Venn diagram (from left to right) represent the number of differentially regulated genes: found only in pEt_20 treatment (left), common to both groups (venn diagram intersection), only in imipenem treatment (right), respectively. b The three independent heat maps depict genes from the corresponding groups in a (as indicated by the respective black arrows). The heat map on the left shows expression data for genes that are only significant in pEt_20 treatment but were shown in conjunction with the expression data from imipenem treatment (not differentially regulated, i.e., Log₂-fold change in the range between −1 and +1). The heat map in the center depicts significant genes that are shared by both treatment groups. The heat map on the right shows genes that are only significant in imipenem treatment along with Log₂-fold change from pEt_20 treatment (not differentially regulated but shown for comparison). The row color side bars on all three heat maps represent the KEGG categories that the genes represent