Fig. 7
In vivo efficacy in CLP and P. aeruginosa lung infection models. a–c Cecal ligation and puncture (CLP) model; d–f P. aeruginosa (PA14)-caused lung infection model. a Survival of mice in the sham group that went through surgery but without CLP, in the CLP group without treatment, in the gentamicin-treated CLP group (Gen, 10 mg/kg mouse body weight at 1 h and 6 h post infection), and in the pEt_20-treated group (pEt_20, 25 mg/kg mouse body weight at 1 h and 6 h post infection; each dose lower than LD5 value) (n = 6, p = 0.0112 (<0.05), Log-rank test). b Microbial counts (CFUs) in the blood and peritoneal fluid (c) at 24 h after CLP. WT: control group without surgery or treatment. Means ± s.d., n = 3. d Survival of mice with P. aeruginosa (PA14)-caused lung infection without and with pEt_10 or imipenem treatment (8 mg/kg mouse body weight at 1, 6, and 25 h post infection) (n = 6, p = 0.0214 (<0.05), Log-rank test). e Bacterial counts (CFUs) in the blood and the lung tissues (f). Means ± s.d., n = 3. One-way ANOVA (Tukey’s post hoc); *p < 0.05; **p < 0.01. Control group: without infection or treatment
