Fig. 2
From: Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression
The Eya3–PP2A association is disrupted by the Eya3 H79A mutation in cells, and can be observed in the endogenous context. a Western blot analysis on whole cell lysates demonstrates levels of exogenous Eya3, as detected with an anti-Eya3 antibody, expressed in HEK293FT cells transfected with empty vector (EV), Flag-Eya3WT or Flag-Eya3H79A. Molecular weight markers in kilodalton are labeled on the side of western blots. b Representative images of proximity ligation assay (PLA) demonstrating the association between Eya3 and PP2A-C in HEK293FT cells transfected with EV, Eya3WT, or Eya3H79A are shown, scale bar: 20 μm. c Quantification of relative PLA/DAPI signal ratio in b, EV group is set to 1 and p-value is calculated using one-way ANOVA followed by Tukey test, ***p < 0.001, error bars: standard deviations (SD), n = 5. d Representative PLA images examining the endogenous Eya3–PP2A-C association in multiple mouse and human breast cancer lines: 66cl4, Met1, MCF7, and MDA-MB-231 are shown, scale bar: 20 μm
