Fig. 3
From: Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression
All Eya family members interact with PP2A, and the N-terminal domain (NTD) of Eya3 is the main contributor to the PP2A interaction. a HEK293FT cells were transfected with EV, Flag-fused human Eya1 (hEya1), hEya2, hEya3, or hEya4. IP against Flag demonstrates the levels of PP2A-C subunit associated with Flag-hEyas. Molecular weight markers in kilodalton are labeled on the side of western blots. b Thr phosphatase activity assay was conducted using hEyas immunoprecipitated from transfected HEK293FT cells. All phosphatase activity was normalized to the amount of protein used in the assay. hEya3 Thr phosphatase activity was set to 100%. c HEK293FT cells were transfected with EV, Flag-fused wildtype (WT), NTD, or C-terminal domain (CTD) Eya3. IP against Flag demonstrates the levels of PP2A-C subunit associated with different domains of Flag-Eya3. d Thr phosphatase activity assay was conducted using different domains of Eya3 immunoprecipitated with a Flag antibody from transfected HEK293FT cells. Eya3 WT Thr phosphatase activity was set to 100%. Data shown are mean ± SD from three independent experiments. In the phosphatase activity assay, the p-value is calculated using one-way ANOVA followed by Tukey test, **p < 0.01, and ***p < 0.001
