Fig. 5

Attenuated infectivity on endothelial progenitor cells is accompanied by innate immune activation and interferon sensitivity. Viral replication of different ZIKV strains in human embryonic stem cell-derived endothelial progenitor cells (hESC-derived EPCs) was determined by a qPCR (n = 6) and b plaque assay (n = 9) (results are combined from three experiments). c NanoString nCounter analysis was performed on infected EPCs at 24 h post infection (n = 3) using both the Immunology and Inflammation prebuilt panels. Counts obtained for CXCL10, IFNB1, and IFIT2 were normalized to GAPDH counts. d Percent inhibition of virus replication as determined by viral RNA qPCR on Vero culture supernatant after 48 h of infection with co-treatment at different concentrations of IFN-λ1 (n = 3 per data point, representative data of two experiments). See also Supplementary Fig. 3. Error bars represent s.d. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 in unpaired t test. n.s. not significant