Fig. 4
Enhanced αM integrin-dependent crawling of MT4-MMP-null patrolling monocytes on CCL2-inflamed endothelium. a Representative intravital microscopy images of CD115+/Ly6C– patrolling monocytes (CD115 in red and Ly6C in green) crawling on the CCL2-inflamed endothelium in the cremaster muscle of wild-type (MT4+/+) and MT4-MMP-null (MT4–/–) mice; the recording was performed in the presence of anti-Itgam blocking antibody (M1/70) or IgG isotype control. Arrowheads, arrows, and dots respectively indicate individual patrolling monocytes, blood flow, and monocyte trajectory. Time of recording is indicated. b The graph shows the numbers of crawling patrolling monocytes recorded in a in every venule from five independent mice per genotype and condition in two independent experiments. c Quantification of CD115+Ly6G- rolling (left) and adherent (right) monocytes in the CCL2-inflamed endothelium in the cremaster muscle of wild-type (MT4+/+) and MT4-MMP-null (MT4–/–) mice. n = 8 mice per genotype in two independent experiments. Data were tested by one-way ANOVA followed by Bonferroni’s post test in b and by two-tailed Student’s t-test in c. Results are expressed as mean ± SEM.*p < 0.05, **p < 0.01, and ***p < 0.001
