Fig. 5
Core symbiont strains possess complete or near complete pathways for N-recycling and amino acid biosynthesis. Heatmap illustrates the proportion of genes present from each N-metabolic pathway across distinct symbiont strains. Coding capacities for strains were inferred from 14 fully sequenced cultured isolate genomes (symbionts from C. varians and C. rohweri) and 11 draft genomes (assembled from C. varians colony PL010 metagenome; identified by the term “Bin” within their names). The maximum likelihood phylogeny of symbiotic bacteria on the left was inferred using an alignment of amino acids encoded by seven phylogenetic marker genes obtained from symbiont genomes, and branch colors are used to illustrate distinct bacterial orders. Red asterisk for urea recycling in the Cephaloticoccus-like Opitutales bin (7-1) indicates that urease genes from the PL010 metagenome binned to Opitutales, but not to the draft genome for the dominant strain. When combined with the likely presence of just one Opitutales strain within the PL010 microbiome (see Results), it is likely that a completely assembled genome would encode all urease genes. Black asterisk for arginine indicates that we examined the completeness of arginine biosynthesis based on the pathway with glutamate as a precursor
