Fig. 4
From: Vesicular glutamate release from central axons contributes to myelin damage
Myelin injury is NMDA-receptor-mediated. a CAP recordings from adult rat RON showing elevated functional recovery from ischemia following 120 min MK-801 pre-treatment. b Ischemia-evoked loss and recovery of function following different periods of MK-801 pre-treatment (insert: data summary). ANOVA with Holm−ŠÃdák post test. P values: 90 min **0.002; 120 min ***0.0001; 165 min ***0.0000. c 120 min pre-treatment with selective NMDA receptor GluN2C/D blockers QNZ-46 (P = 0.0013) or PPDA (P = 0.0009), or the TRPA1 blocker A967076. d Oligodendrocyte cytoplasmic domain (PLP-GFP mouse, arrows) is distinct from the myelin domain (FM: fluoromyelin red) in live adult mouse RON. Highlighted square shown in insert as rendered stack. e Myelin structure is protected from ischemic damage by 120 min pre-treatment with QNZ-46, imaged via FM vital staining. f–h Ischemia evoked myelin swelling (P = 0.000 in aCSF) is prevented by 120 min QNZ-46 pre-treatment (P = 0.047 vs. aCSF). Scale = 10 μm
