Fig. 6
From: Vesicular glutamate release from central axons contributes to myelin damage
QNZ-46 is absorbed by myelin. a QNZ-46 emission shows accumulation and retention in adult rat RON. b Confocal imaging of vital QNZ-46 fluorescence (120 min treatment) in adult corpus callosum (left) and the white matter–gray matter border (right). c Vital QNZ-46/FM co-staining of myelinated axon profiles. d, e Vital QNZ-46/FM co-staining in brain slice (d) and RON (e) following systemic QNZ-46 injection 4 h pre-sacrifice. f 60 min QNZ-46 pre-treatment + 60 min wash-out is functionally protective of adult rat RON (ANOVA with Holm−ŠÃdák post test; P = 0.037). g Systemic QNZ-46 pre-treatment is functionally protective of mouse RON perfused with aCSF (blue, P = 0.047), and is not significantly different when QNZ-46 is included in the bath (green). Note, proportional recovery was similar to that found in Fig. 3c (insert). Merged images recolored for clarity. Scale bars = 5 μm
