Fig. 3
From: Functionally distinct and selectively phosphorylated GPCR subpopulations co-exist in a single cell
PKA- and GRK-pβ2AR display minimal overlap at the single-molecule level. a Schematic of single-molecule pulldown (SiMPull) assay. b Representative SiMPull images of a fusion mCherry-mYFP protein, total β2AR, PKA-phosphorylated β2AR, and GRK-phosphorylated β2AR pulled down from cell lysates expressing mCherry-β2AR and mYFP-β2AR at 1:1 ratio. Scale bar, 5 μm. Representative of n = 8, 10, 7, and 9 images, respectively, three independent experiments. c Quantification of overlap percentage between mCherry and mYFP from images in b (n = 8, 10, 7, and 9, respectively). Error bars denote s.d.; multiplicity adjusted P values are computed by one-way ANOVA followed by Tukey’s test between indicated groups
