Fig. 6
From: Functionally distinct and selectively phosphorylated GPCR subpopulations co-exist in a single cell
β2AR is necessary for ISO-induced upregulation of LTCC Cav1.2 activity in hippocampal neurons. a Representative single-channel recordings of LTCC Cav1.2 currents in hippocampal neurons from wild type (WT), β1AR knockout (KO) and β2AR KO mice at 7–14 DIV after depolarization from −80 to 0 mV without (black traces) and with stimulation of 1 μM ISO (red traces) in the patch pipette. b Data in a were quantified and plotted. The ISO-induced increases in LTCC overall channel activity (nPo) were detected in WT and β1AR KO neurons, but absent in β2AR KO neurons (n = 8, 11, 4, 6, 20, and 11 cells, respectively). Error bars denote s.e.m., exact P values are computed by Mann–Whitney test
