Fig. 2
GFP-OsKCH2(289-767) moves on the microtubule with minus-end-directed processive motility. a Schematic diagrams of the full-length OsKCH2 and GFP-OsKCH2(289–767). b Coomassie-stained SDS-PAGE of purified recombinant GFP-OsKCH2(289–767). c Schematic diagram of the ensemble microtubule gliding assay. d Micrograph montage showing the motion of polarity-marked microtubules (red) glided by surface-immobilized GFP-OsKCH2(289–767). Arrowheads (brown, white, and yellow) indicate the plus ends of three different microtubules. e Schematic diagram of the single-molecule motility assay for observing the movement of individual GFP-OsKCH2(289–767) molecules on single microtubules. f Kymographs of single GFP-OsKCH2(289–767) molecules (green) moving processively toward the minus end of single polarity-marked microtubules (red). g Velocity histogram of single GFP-OsKCH2(289–767) molecules. Red line indicates a Gaussian fit to the velocity histogram. h Run-length histogram of single GFP-OsKCH2(289–767) molecules. Red line indicates a single-exponential fit to the run-length histogram. Scale bars: 1 min (vertical) and 5 µm (horizontal)
