Fig. 6
From: TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A
Loss of TRiC reduces RRS and protection against UV damage. a TCP1 loss reduces RNA synthesis recovery following UV-C irradiation. VH10-hTert cells were transfected with the indicated siRNAs and UV-C irradiated (10 J/m2). RNA synthesis was measured by means of EU incorporation at the indicated time points after UV. RNA synthesis levels were normalized to those in non-irradiated cells, which were set to 100%. Data represent the mean ± SEM of four independent experiments. b Expression of CSA-FLAG 8M shows reduced RNA synthesis recovery as compared to expression of CSA-FLAG WT. As in a, except that CS3BE-SV40 cells expressing CSA-FLAG WT or CSA-FLAG 8M were used. Data represent the mean ± SEM of four independent experiments. c TCP1 loss renders cells hypersensitive to UV damage. VH10-hTert cells were transfected with the indicated siRNAs, UV-C irradiated at the indicated doses and 72 h later assayed for viability using alamarBlue®. Data represent mean ± SEM of four independent experiments. d Expression of CSA-FLAG 8M in CS3BE-SV40 cells fails to rescue UV-sensitivity. CS3BE-SV40 cells stably expressing CSA-FLAG WT or CSA-FLAG 8M were UV-C irradiated and clonogenic survival was measured. Data represent mean ± SEM of three independent experiments. e CSA WT, but not CSA ΔN, complements the Illudin S sensitivity of CSA knockout (KO) U2OS cells. The indicated cells were treated with different concentrations of Illudin S and clonogenic survival was determined. Data represent mean ± SEM of three independent experiments
