Fig. 1
From: Light-triggered enzymatic reactions in nested vesicle reactors
UV-responsive nested vesicles can be created via phase-transfer. a Chemical structure of DC89PC and the UV-catalysed polymerisation of diacetylene molecules. b Cartoon of UV-responsive nested vesicle mechanism. Photo-responsive vesicles and the enzyme β-galactosidase are co-encapsulated within POPC GUVs created via phase-transfer. Upon illumination with UV-C, photopolymerisation of the inner compartment membranes results in FDG release, leading to its catalysis by β-galactosidase forming free fluorescein. c Optical microscopy of UV-responsive vesicles before UV irradiation and 30 min after UV irradiation. Scale bar in all images, 25 μm. d Comparison of mean fluorescence of UV-responsive nested vesicles before and after 10 min irradiation and 30 min wait. Controls are left for 40 min without application of UV. Error bars represent 1 s.d. (n = 71/79 for before/after UV). e Histogram of vesicle population in C before and after UV
