Table 1 Hydrolytic kinetic resolution of different substrates using (S)-selective and (R)-selective AFEST mutants
Substrate | Enzyme | Reaction time (min) | Conv. (%) | eep (%) | E |
---|---|---|---|---|---|
rac-1 | WT | 10 | 23 | 70 (R) | 7.4 (R) |
rac-1 | 6A8 | 10 | 20 | >99 (S) | >100 (S) |
rac-1 | 4D11 | 6 | 18 | 97 (S) | 80 (S) |
rac-1 | 4E12 | 20 | 47 | 95 (R) | 74 (R) |
rac-2 | WT | 20 | 22 | 40 (R) | 3.0 (R) |
rac-2 | 6A8 | 30 | 24 | 79 (S) | 11 (S) |
rac-2 | 4D11 | 10 | 21 | 94 (S) | 35 (S) |
rac-2 | 4E12 | 20 | 26 | 82 (R) | 13 (R) |
rac-3 | WT | 20 | 18 | 81 (R) | 12 (R) |
rac-3 | 6A8 | 20 | 22 | 92 (S) | 31 (S) |
rac-3 | 4D11 | 10 | 34 | 93 (S) | 42 (S) |
rac-3 | 4E12 | 20 | 38 | 75 (R) | 11 (R) |
rac-4 | WT | 6 | 27 | 37 (R) | 3.0 (R) |
rac-4 | 6A8 | 60 | 29 | 68 (S) | 7.4 (S) |
rac-4 | 4D11 | 3 | 25 | 95 (S) | 88 (S) |
rac-4 | 4E12 | 20 | 22 | 68 (R) | 6.7 (R) |
rac-5 | WT | 10 | 24 | 46 (R) | 3.6 (R) |
rac-5 | 6A8 | 60 | 18 | 78 (S) | 10 (S) |
rac-5 | 4D11 | 3 | 25 | 95 (S) | 52 (S) |
rac-5 | 4E12 | 10 | 26 | 64 (R) | 6.2 (R) |