Fig. 3
From: Apolipoprotein AI prevents regulatory to follicular helper T cell switching during atherosclerosis
Blocking ICOSL reduces exTreg Tfh cells and atherosclerosis. a–e LT-ApoE−/− mice were fed a western diet for 9 weeks and then were injected with antibody against ICOSL or isotype i.p. (100 μg per mouse, twice per week) for 6 weeks. Graphs show the numbers of exTreg (a), exTreg Tfh cells (b) in aorta, PaLN and spleen, germinal center B cells in PaLN (c) and current Treg cells in aorta and spleen (d). e Oil Red O stain of aortic roots from the above mice. Images are representative section from each group of mice. Each individual dot in the graph is the average of at least 4 sections per aorta per mouse. Scale bars = 100 μm. Results are expressed as mean ± s.e.m. from two independent experiments (For aorta, n = 6 (isotype) and n = 10 (αICOSL). For PaLN and spleen, n = 8 (isotype) and n = 10 (αICOSL). For aortic roots, n = 8 (isotype) and n = 11 (αICOSL). Statistical significant differences were at *P < 0.05, **P < 0.01, and ***P < 0.001 (unpaired Student’s t-test)
