Fig. 8

ApoAI administration increases IL-2Rα expression and reduces IL-6 production and Bcl6 expression during atherogenesis. a Expression of IL-2Rα (CD25) and IL-6Rα (CD130) on current Treg in the aorta of LT-ApoE^−/− western diet-fed mice with and without ApoAI treatment or chow controls. b Plasma levels of IL-6 in the mice described above. c Flow cytometry plots and graphs showing the percentages and numbers of CXCR5⁺Bcl6⁺ Tfh in exTreg cells of above mice. Plots are gated on CD44⁺RFP⁺YFP⁻ CD4 T cells. d Naive CD4 T cells from ApoE^−/− mice were isolated and stimulated with αCD3/CD28 and TGFβ to induce Treg in vitro with or without the addition of oxLDL or ApoAI. Flow cytometry plots and graphs showing the percentages of induced Treg following 4 days in culture. Results are expressed as the mean ± s.e.m. from one of two independent experiments (n = 6 (Chow and WD/ApoAI), n = 7 (WD)) (a), mean ± s.e.m. from 3 different experiments (n = 18 (Chow), n = 17 (WD) and n = 13 (WD/ApoAI)) (b), the mean ± s.e.m. from two independent experiments (n = 11 (Chow and WD) and n = 10 (WD/ApoAI) (c), and the mean ± s.e.m. from one of two experiments (n = 4) (d). Statistical significant differences were at *P < 0.05, **P < 0.01 and ***P < 0.001 (one-way ANOVA)