Fig. 1 | Nature Communications

Fig. 1

From: RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia

Fig. 1

Differential expression of RNA:m5C, RCMTs and hnRNPK in 5-AZA-sensitive and 5-AZA-resistant leukaemia cells and the binding of hnRNPK to unmethylated and cytosine-methylated RNA. a Western blot analysis of expression of RCMTs, hnRNPK and other proteins in the 5-AZA-sensitive OCI-M2 and SC leukaemia cells and the 5-AZA-resistant M2AR and SCAR leukaemia cells. b Dot blot analysis of 5-methylcytosine (m5C) in RNA and DNA from OCI-M2 and M2AR cells. c Dot blot analysis of 5-methylcytosine (m5C) in RNA and DNA from SC and SCAR cells. d, e Visualization and measurement of the binding of purified recombinant hnRNPK to the unmethylated and cytosine-methylated fluorescein (FAM)-labelled RNA oligos by an antibody-coupled bead assay

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