Fig. 2 | Nature Communications

Fig. 2

From: RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia

Fig. 2

Distinct functional hnRNPK/RCMT complexes required for leukaemia cell survival. a RNase digestion-coupled immunoprecipitation (IP) and co-immunoprecipitation (co-IP) to examine the interactions between hnRNPK and RCMTs, including DNMT2, NSUN3, NSUN1 and NSUN2, in OCI-M2 cells. b Measurements of the expression of hnRNPK, DNMT2, NSUN3 and other proteins in SC leukaemia cells treated with control siRNAs and the siRNAs targeting HNRNPK, DNMT2 and NSUN3 at day 3. c Measurements of the expression of NSUN1, NSUN2 and other proteins in SC leukaemia cells treated with control siRNAs and the siRNAs targeting NSUN1 and NSUN2 at day 3. d Measurements of the growth of SC leukaemia cells treated with control siRNAs and siRNAs targeting hnRNPK and individual RCMTs by MTT assay at day 3. Data are presented as the mean ± SEM of n = 3 independent samples. *P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t test

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