Fig. 4

RNA/hnRNPK-mediated selective interactions between lineage-determining TFs and chromatin modifiers. a Western blotting, IP with GATA1 and SPI1/PU.1 antibodies and co-IP with antibodies against various DNA and histone modifiers in OCI-M2 and SC cells. b Examination of the effects of 5-AZA on the interactions between SPI1/PU.1 and DNMT1 as well as EZH2 by co-IP in OCI-M2 cells treated with 1 μM 5-AZA at various time points. c Examination of the effects of 5-AZA on the interaction between SPI1/PU.1 and TET2 by co-IP in SC cells treated with 1 μM 5-AZA at various time points. d Examination of the RNA-dependence of the interaction between GATA1 and TET2 and the interactions between SPI1/PU.1 and DNMT1 as well as EZH2 by RNase digestion-coupled co-IP in OCI-M2 cells. e Examination of the RNA-dependence of the interaction between SPI1/PU.1 and TET2 by RNase digestion-coupled co-IP in SC cells. f Identification of hnRNPK as a direct binder of both GATA1 and TET2 by IP with GATA1 antibody and co-IPs with antibodies against various RNA-binding proteins, such as hnRNPA1 and AUF1, in OCI-M2 cells. g A schematic illustration of the nature of the interactions between hnRNPK and GATA1 as well as TET2 in OCI-M2 cells