Fig. 6 | Nature Communications

Fig. 6

From: RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia

Fig. 6

Identification of unique interactions between RNA-pol-II CTD-S2P and NSUN1 as well as BRD4 in 5-AZA-resistant leukaemia cells. a Examination of the protein expression of the components of active RNA-pol-II complex, BRD4 and BRD2 in the 5-AZA-sensitive OCI-M2 and SC cells and the 5-AZA-resistant M2AR and SCAR cells by western blotting. b Examination of the effects of 5-AZA on the interactions between hnRNPK and RNA-pol-II CTDS2P as well as GATA1 in OCI-M2 and M2AR cells by IP and co-IP. c Elucidation of a unique 5-AZA-resistant interaction between RNA-pol-II CTD-S2P and NSUN1 in the 5-AZA-resistant M2AR cells by IP and co-IP. d Elucidation of unique interactions between BRD4 and RNA-pol-II CTD-S2P as well as NSUN1 in the 5-AZA-resistant M2AR cells by IP and co-IP and the effects of JQ1 on these interactions

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