Fig. 7 | Nature Communications

Fig. 7

From: RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia

Fig. 7

Distinct NSUN1/BRD4-associated active chromatin structures in 5-AZA-restsant leukaemia cells. a Elucidation of the subcellular localization of nascent RNA, NSUN1 and BRD4 by 5-ethynyluridine (EU)-clicking chemistry-coupled STED confocal microscopy in OCI-M2 and M2AR cells with 0.3 mM EU for 5 h. b Elucidation of the subcellular localization of nascent RNA, NSUN1 and RNA-pol-II CTD-S2P by EU-clicking chemistry-coupled STED confocal microscopy in OCI-M2 and M2AR leukaemia cells treated with 0.3 mM EU for 5 h. c Demonstration of markedly increased co-localization of NSUN1 and BRD4 by proximity ligation and rolling circle amplification (PL-RCA) in OCI-M2 and M2AR cells. d Demonstration of markedly increased co-localization of NSUN1 and RNA-pol-II CTD-S2P by proximity ligation and rolling circle amplification (PL-RCA) in OCI-M2 and M2AR cells

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