Fig. 4 | Nature Communications

Fig. 4

From: An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations

Fig. 4

Molecular level localization mapping using the reference molecular context maps. a The schematic overview of the MS microscopy approach to assess the queried protein localization using our reference interaction context. b The polar plot shows the location of query protein observed by MS microscopy. Each sector represents one subcellular location defined by our reference database. The color assigned to each of the localization is based on the annotation frequency (Pink: 0–0.5; Yellow: 0.5–0.75; Green: 0.75–1). c, d The PPI network obtained from BioID and AP-MS are shown separately. The localization of prey proteins was verified by CellWhere database. Node color scheme coordinates the observation localization from Fig. 4b

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